High-density oligonucleotide arrays
Web23 de dez. de 2002 · We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E 2) on the transcriptomes from the medial basal hypothalamus and other brain regions of E 2-treated (10 μg) adult female mice. Our results have revealed several unanticipated gene regulations. Webnucleotide polymorphism array, spectral karyotyping, tiling array Most human cancers are characterized by genetic instabilities. These instabilities manifest themselves as a series of genetic alterations, including discrete mutations and chromosomal aberrations. With the human genome deciphered, high-throughput
High-density oligonucleotide arrays
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Web19 de set. de 2003 · High-density oligonucleotide arrays (1, 2) have revolutionized the study of gene expression.The technology enables researchers to detect and quantify tens of thousands of transcripts in a single experiment and has become a standard for the discovery of gene functions, drug evaluation, pathway dissection, and classification of clinical … WebAbstract. Motivation: High-density oligonucleotide arrays (GeneChip, Affymetrix, Santa Clara, CA) have become a standard research tool in many areas of biomedical …
Web18 de mai. de 2007 · In spotted arrays, complementary DNA or long oligonucleotide probes are printed onto the substrate. The two messenger RNA samples of interest are reverse transcribed into complementary DNA, labelled with a different fluorescent dye (green cyanine 3 or red cyanine 5) and mixed together, before being washed over the slide and …
Web24 de jul. de 2015 · The methods for preparing DNA chips combine photochemistry and photolithography with solid-phase DNA synthesis chemistry to generate a high-density oligonucleotide probe array in situ [6,7,8]. These two-dimensional arrays containing hundreds or thousands of oligonucleotide probes provide a powerful DNA sequence … WebAbstract: Genomic DNA-based probe selection by using high density oligonucleotide arrays has recently been applied to heterologous species (Xspecies). With the advent of this new approach, researchers are able to study the genome and transcriptome of a non-model
WebWe explored the utility of high-density oligonucleotide arrays {DNA chips) for obtaining sequence information from homolo gous genes in closely related species.
WebAlthough oligonucleotide probes are often used in "spotted" microarrays, the term "oligonucleotide array" most often refers to a specific technique of manufacturing. … canned sausage gravy brandsWeb1 de jan. de 1996 · Refereed paperHigh-density oligonucleotide arrays. We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniaturize conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a … fix print spooler windows 10 en francaisWeb1 de jan. de 1997 · Oligonucleotide arrays are also used for DNA sequencing and genotyping (Gingeras et al., 1998), which is a promising application of the high-density oligonucleotide hybridization platform. ... canned scripts meaningWeb1 de jan. de 2002 · The leading arrays in the category of high-density oligonucleotide arrays are manufactured by Affymetrix and utilize the combinatorial synthesis principle. The arrays are designed by using a light-directed chemical synthesis process that creates a series of photolithographic masks to define chip exposure sites, followed by specific … canned scriptsWebAnother study with Arabidopsis used high-density oligonucleotide arrays that cover the entire genome. More than 10 times more transcripts were found than predicted by ESTs [ … fix print spooler windows 10 telechargerWeb1 de jan. de 2002 · High-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. Photolithographic DNA synthesis has enabled the large-scale production of GeneChip probe arrays containing hundreds of thousands of oligonucleotide sequences on a glass … fix print spooler win 10Web17 de jun. de 2008 · Affymetrix high density oligonucleotide expression arrays are widely used across all fields of biological research for measuring genome-wide gene expression. An important step in processing oligonucleotide microarray data is to produce a single value for the gene expression level of an RNA transcript using one of a growing number … fix privacy error in chrome