WebThis chapter describes the methods for osteoclast formation in vitro in the presence and absence of osteoblasts, and for pit-formation assay using dentine slices and osteoclasts formed in vitro. These culture systems have made it possible to investigate each step of osteoclast development and function separately. Web2 days ago · WHITEHORSE, YT, April 12, 2024 /CNW/ - Minto Metals Corp. ("Minto" or the "Company") is pleased to report final results from its 2024 exploration d...
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WebNov 12, 2024 · The resorption pit assay was conducted following the methods described by Lu et al . RAW264.7 cells (1×10 5 cells/well) were seeded at 37°C on a 24-well Corning Osteo Assay Surface well plate (Corning, Inc.), and were pretreated with PCA (8 mM) for 2 h followed by the addition of 100 ng/ml RANKL. After 5 days of incubation, all remaining ... WebJun 13, 2024 · TRAP staining, the pit formation assay, and the actin ring formation assay were performed as previously described (Iezaki et al., 2016). Briefly, cells were cultured on bone slices and subsequent fixation with 4% paraformaldehyde, followed by permeabilization with 0.5% Triton X-100. For the pit assay, the bone slices were … i know artificial intelligence
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WebMar 1, 2011 · The pit assay is the principal method for evaluating osteoclast function by measuring hydroxyapatite resorption in vitro. However, the pit assay requires time and … WebApr 7, 2024 · Resorption Pit Assay To observe the bone-resorptive function of osteoclasts in vitro, a resorption pit assay was performed. BMMs were then inoculated into 6-well plates, followed by five days of incubation using the induced medium. WebJun 16, 2024 · In this protocol, we describe a simple pit assay method using calcium phosphate (CaP) coated cell culture plates, which can be easily visualized and quantified. Osteoclast precursors derived from human peripheral blood mononuclear cells (PBMCs) were cultured on the coated plates in the presence of osteoclastogenic stimuli. i know a secret